qRT-PCR with saliva samples
نویسندگان
چکیده
منابع مشابه
Evaluation of bacterial internalization using qRT-PCR.
1Bacterial resistance to antibiotics currently represents a topic of high interest. Unfortunately, studies addressing their adaptation and multidrug tolerance latency are not sufficient for a comprehensive description of the pathogen-host molecular interaction mechanisms (interactome) and the bacterial internalization into human cells, which leads to modified human genes. Bacterial persisters a...
متن کاملComparison of Prognostic Gene Profiles Using qRT-PCR in Paraffin Samples: A Retrospective Study in Patients with Early Breast Cancer
INTRODUCTION Gene profiling may improve prognostic accuracy in patients with early breast cancer, but this technology is not widely available. We used commercial assays for qRT-PCR to assess the performance of the gene profiles included in the 70-Gene Signature, the Recurrence Score and the Two-Gene Ratio. METHODS 153 patients with early breast cancer and a minimum follow-up of 5 years were i...
متن کاملRT ProfilerTM PCR Arrays: Pathway-Focused Gene Expression Profiling with qRT-PCR
This paper evaluates the performance of the newest technique for monitoring the expression of a panel of pathwayor disease-specific genes: the RT2 Profiler PCR Array System from SA Biosciences. The PCR Array System combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray. The PCR Array is a 96or 384-well plate contain...
متن کاملCollecting saliva samples by mail.
Collecting saliva samples by mail can serve numerous purposes in epidemiologic research. The objectives of this study were to assess what proportion of participants in a mail survey would provide a saliva sample and whether incentives could improve participation. In 1995, 2,994 students, faculty, and staff members of Geneva University, Geneva, Switzerland, were randomized to receive, together w...
متن کاملGenomic quantitative real-time PCR proves residual disease positivity in more than 30% samples with negative mRNA-based qRT-PCR in Chronic Myeloid Leukemia
Imatinib mesylate (IM) is the first line therapy against Chronic Myeloid Leukemia, effectively prolonging overall survival. Because discontinuation of treatment is associated with relapse, IM is required indefinitely to maintain operational cure. To assess minimal residual disease, cytogenetic analysis is insensitive in a high background of normal lymphocytes. The qRT-PCR provides highly sensit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2012
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113921